Wu Zhenqi*,Wang Guibang,Yan Feng,et al.The experimental research of Qingzao-Jiufei decoction and its composition under the influence of immune inflammation of mice infected with mycoplasma pneumoniae [J].,2018,40(5):442-446.[doi:DOI: 10.3760/cma.j.issn.1673-4246.2018.05.014]
清燥救肺汤及其分解剂对肺炎支原体感染模型小鼠免疫炎症的影响
- Title:
- The experimental research of Qingzao - Jiufei decoction and its composition under the influence of immune inflammation of mice infected with mycoplasma pneumoniae
- Keywords:
- Mycoplasma pneumoniae; Qing Zao Jiu Fei Tang; Inflammation; T-lymphocyte subsets; Immunocompetence; Mice
- 摘要:
- 目的 观察清燥救肺汤及其分解剂对肺炎支原体(mycoplasma pneumonia, MP)感染小鼠血清IL-10、TNF-a水平，CD4＋、CD8＋T细胞水平及CD4＋/CD8＋比值变化影响，探讨其抗MP感染的作用机制。方法 将144只BALB/c小鼠按随机数字表法分为正常组、模型组、全方组、分解Ⅰ组、分解Ⅱ组及阿奇霉素组，每组24只。除正常组外，其余各组小鼠制备MP感染模型。造模成功后，全方组灌胃清燥救肺汤混悬液1 g/ml、分解I组灌胃分解剂Ⅰ混悬液0.61 g/ml、分解Ⅱ组灌胃分解剂Ⅱ混悬液0.39 g/ml，1次/d，连续14 d。阿奇霉素组灌胃阿奇霉素混悬液6 mg/ml，1次/d，连续3 d后暂停4 d继续给药，分别于造模后第3、7、10、14天进行取材，摘小鼠眼球取血，采用ELISA法测定各组小鼠血清IL-10和TNF-a水平变化；采用流式细胞术检测小鼠脾脏灌洗液中CD4＋、CD8＋T细胞水平并计算CD4＋/CD8＋比值。结果 造模后第14天，与模型组比较，全方组CD4＋T细胞[(24.50±1.41)比(22.08±1.99)]、IL-10[(18.15±0.36)比(8.75±0.16)]表达升高(P＜0.05)；CD8＋T细胞[(7.29±1.23)比(9.13±1.14)]、TNF-a[(28.32±1.90)比(37.97±1.71)]表达下降(P＜0.05)；CD4＋/CD8＋比值升高(P＜0.05)；分解Ⅰ组CD8＋T细胞[(7.50±1.45)比(9.13±1.14)]、TNF-a[(33.48±1.08)比(37.97±1.71)]表达均下降(P＜0.05)；分解Ⅱ组CD4＋T细胞[(23.63±1.10)比(22.08±1.99)]、IL-10[(17.82±0.63)比(8.75±0.16)]表达升高(P＜0.05)。结论 清燥救肺汤可抗MP感染，其作用机制可能与其调节细胞免疫作用有关。
- Abstract:
- Objective To observe the effect of Qingzao-Jiufei decoction and its composition on the levels of IL-10,TNF-a, CD4＋cells, CD8＋T cells and the change of CD4＋/CD8＋ratio in the serum to explore the mechanism of anti-MP infection. Methods The 144 SPF grade BALB/c mice were randomly divided into the normal group, the model, the whole decoction group, the composition Ⅰ group, the composition Ⅱgroup with the method of random digital table, and the azithromycin group and every group had 24 mice. The following five groups were treated with infection of MP model. After successful modeling, the rats in the whole decoction group were treated by gavage with 1 g/ml Qingzao-Jiufei decoction. The rats in the composition Ⅰ group were treated by gavage with 0.61 g/ml composition Ⅰ decoction. The rats in the composition Ⅱ group were treated by gavage with 0.39 g/ml composition Ⅱ decoction. And the three groups were administered for 14 days. The rats in the azithromycin group were treated by gavage with azithromycin suspension once a day. After three days, the drug was stopped. Each group was infected on 3,7,10,14 day. Then mice were sacrificed, with the method of taking the eye of the mice to take blood. The level of IL-10, TNF-a in the mice blood with the method of ELISA were detected. The level of CD4＋, CD8＋T in the rinsing solution of mice spleen with the method of FCM and the CD4＋/CD8＋ ratio were detected. Results Compared with the model group, the expressions of CD4＋T cells (24.50 ± 1.41 vs. 22.08 ± 1.99) and IL-10 (18.15 ± 0.36 vs. 8.75 ± 0.16) in the whole decoction group significantly increased. The expressions of CD8＋T cells (7.29 ± 1.23 vs. 9.13 ± 1.14) and TNF-a (28.32 ± 1.90 vs. 37.97 ± 1.71) significantly decreased (P<0.05), and the level of CD4＋/CD8＋ratio increased. In the composition Ⅰ group, the expressions of CD8＋T cells (7.50 ± 1.45 vs. 9.13 ± 1.14) and TNF-a (33.48 ± 1.08 vs. 37.97 ± 1.71) significantly decreased (P<0.05). In the composition Ⅱ group, the expressions of CD4＋T cells (23.63 ± 1.10 vs. 22.08 ± 1.99) and IL-10 (17.82 ± 0.63 vs. 8.75 ± 0.16) significantly increased (P<0.05). Conclusions The mechanism of Qingzao-Jiufei decoction on anti-MP infection may be related to the regulation of the cellular immune.
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备注/Memo
基金项目：国家自然科学基金(81373687)；辽宁省科技厅项目(2014020044) 通信作者：吴振起，Email: zhenqiwu@163.com
更新日期/Last Update:
2018-05-12