Li Hongbing,Yao Xuan,Xie Rongrong,et al.Effect of Tangshen-Huazhuo recipe on the levels of serum transforming growth factor β1 and platelet derived growth factor in patients with IV stage of diabetic nephropathy[J].,2014,36(10):869-872.[doi:10.3760/cma.j.issn.1673-4246.2014.10.002]
糖肾化浊方对IV期糖尿病肾病患者血清转化生长因子-β1和血小板源生长因子浓度的影响
- Title:
- Effect of Tangshen-Huazhuo recipe on the levels of serum transforming growth factor β1 and platelet derived growth factor in patients with IV stage of diabetic nephropathy
- Keywords:
- Tangshen-Huazhuo Recipe; Diabetic nephropathy; Transforming growth factor b1; Platelet derived growth factor
- 摘要:
- 目的 探讨糖肾化浊方对IV期糖尿病肾病（diabetic nephropathy，DN）患者血清转化生长因子-β1（TGF-β1）和血小板源生长因子（PDGF）浓度的影响。方法 收集2012年6－12月在首都医科大学附属北京同仁医院就诊的IV期DN患者98例，采用随机数字表法将患者分为治疗组（48例）和对照组（50例），均采用常规降糖、降压、调脂和抗凝治疗，停服血管紧张素转化酶抑制药或其他血管紧张素II受体拮抗药。对照组在此基础上口服厄贝沙坦，150 mg/d。治疗组在对照组治疗基础上加服糖肾化浊方。两组治疗后6个月检测血清TGF-β1和PDGF浓度，并观察尿微量白蛋白排泄率（UAER）、血尿素氮（BUN）、血肌酐（Scr）、糖化血红蛋白（HbA1c）、三酰甘油（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）的变化。结果 治疗后，治疗组TGF-β1、PDGF水平[（123.6±21.2）pg/ml、（500.6±130.2）pg/ml]较同组治疗前[（172.5±31.3）pg/ml、（860.9±131.2）pg/ml]明显下降（P＜0.01），且与对照组治疗后[（157.4±39.6）pg/ml、（765.7±161.8）pg/ml]比较，差异有统计学意义（P＜0.01）。治疗后治疗组TG、TC、HDL-C、LDL-C、UAER[（1.72±0.25）mmol/L、（4.56±0.64）mmol/L、（1.56±0.50）mmol/L、（2.46±1.08）mmol/L、（100.73±204.24）mg/min]均优于对照组[（2.09±0.27）mmol/L、（6.11±0.93）mmol/L、（1.36±0.44）mmol/L、（3.32±0.87）mmol/L、（226.24±396.38）mg/min，P＜0.05或0.01]。结论 糖肾化浊方可降低IV期DN患者血清TGF-b、PDGF浓度，抑制或改善肾小球硬化发生、发展过程。
- Abstract:
- Objective To investigate the effect of extract of Tangshen-Huazhuo Recipe(TSHZR) on the serum concentrations of transforming growth factor β1(TGF-β1) and platelet derived growth factor (PDGF) in patients with Ⅳ stage of diabetic nephropathy(DN). Methods From June 2012 to December 2012, 98 patients of Ⅳstage DN in our hospital outpatient were enrolled and randomly divided into treatment group(n＝48) and control group (n＝50) using random number table. All patients received conventional therapies of controlling blood sugar, lipid, blood pressure and anticoagulant therapy. On such basis, the control group was treated by irbesartan, 150 mg/d, and the treated group treated by TSHZR combined with irbesartan,150 mg/d, for 6 months. Serum TGF-b1 and PDGF were determined with ELISA before and after treatment，and urinary albumin excretion rate，HbA1c，serum creatinine，blood urea nitrogen and lipid profiles were examined as well. Results In the treated group, the TGF-β1 was(172.5±31.3), (123.6±21.2)pg/ml, the PDGF was(860.9±131.2), (500.6±130.2)pg/ml before the treatment and after the treatment, respectively. The TGF-b1 and PDGF after the treatment were significantly decreased than those before the treatment(P＜0.01). After the treatment, TGF-β1 and PDGF in the treated group were statistically significant compared to the control group［TGF-β1 is(157.4±39.6)pg/ml, PDGF is(765.7±161.8)］pg/ml, P＜0.01). After the treatment, the treatment group wassuperior to the control group in TG(1.72±0.25)mmol/L, (2.09±0.27)mmol/L, (P＜0.01), TC(4.56±0.64)mmol/L, (6.11±0.93)mmol/L, (P＜0.01), HDL-C(1.56±0.50)mmol/L, (1.36±0.44)mmol/L, (P＜0.01), LDL-C(2.46±1.08)mmol/L (3.32±0.87)mmol/L, (P＜0.05)and UAER(100.73±204.24)mg/min, (226.24±396.38)mg/min, (P＜0.01). Conclusion TSHZR can inhibit the progressive of IV stage of diabetic nephropathy by suppressing TGF-β1 and PDGF expression level.
参考文献/References:
［1］ 陈志宏. 糖尿病肾病发病机制研究进展[J]. 承德医学院学报，2010，27（2）：184-186. ［2］ 中华医学会糖尿病学分会. 中国2型糖尿病防治指南[M]. 北京：北京大学医学出版社，2011：5. ［3］ Mogensen CE. Early diabetic renal involvement and nephropathy[M]. Amsterdam：Elsevier science publishers，1987：306. ［4］ Tammo O，Frank E，Jürgen F. The PDGF family in renal fibrosis[J]. Pediatr Nephrol，2012，27（7）：1041-1050. ［5］ Jiji Inassi，Vijayalakshmy R. Role of Duration of Diabetes in the Development of Nephropathy in Type 2 Diabetic Patients[J]. Nati J Med Res，2013，3（1）：5-8. ［6］ Fraser D，Bruskill N，Ito T，et al. Long-term exposure of proximal tubular epithelial cells to glucose induces transforming growth factor-b1 synthesis via an auto cine PDGF loop[J]. Am J Pathol，2003，163：2565-2574. ［7］ Orhan MD，Giray B，Selda K，et al. The evaluation of some flexural properties of a denture base resin reinforced with various aesthetic fibers[J]. J Mate Sci: Mate Med，2008，19（6）：2343-2349. ［8］ Zdunek M，Silbiger S，Lei J，et al. Protein kinase CK2 mediates TGF-β1stimulated type IV collagen gene transcription and its reversal by estradiol[J]. Kidney Int，2001，60（6）：2097-2108. ［9］ Crean JK，Finlay D，Murphy M，et al. The role of p42/p44 MAPK and protein Kinase B in connective tissue growth factor induced extracellular matrix protein production, cell migration, and actin cytoskeletal rearrangement in human mesangial cells[J]. J Biol Chem，2002，277（46）：44187-44194. ［10］ Kam S，van der Geest RN，Verhagen NA，et al. Secretion of collagen type Ⅳ by human renal fibroblasts is increased by high glucose via a TGF-β independent pathway[J]. Nephrology Dia Transplant，2004，19：1694-1701. ［11］ Taneda S，Hudkins KL，Cui Y，et al. Growth factor expression in a murine model of cryoglobulinemia[J]. Kidney Int，2003，63（2）：576-590.
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更新日期/Last Update:
2014-09-29