Miao Yanfang,Wang Yuxian,Zhang Shengsheng.Effects of Tongxie-Yaofang on visceral sensitivity and expression of 5-HT and SERT in IBS-D model rats[J].,2021,43(12):1209-1214.[doi:10.3760/cma.j.cn115398-20210122-00231]
痛泻要方对腹泻型肠易激综合征大鼠内脏敏感性及5-羟色胺、5-羟色胺转运体蛋白的影响
- Title:
- Effects of Tongxie-Yaofang on visceral sensitivity and expression of 5-HT and SERT in IBS-D model rats
- Keywords:
- Irritable bowel syndrome; Tong Xie Yao Fang; Visceral hypersensitivity; 5-hydroxytryptamine; Serotonin transporter; Rats
- 摘要:
- 目的 探讨痛泻要方对腹泻型肠易激综合征模型大鼠内脏敏感性的影响及其作用机制。方法 将30只雄性1日龄SD大鼠按随机数字表法分为正常组(10只)和造模组(20只)。采用母婴分离＋束缚应激构建腹泻型肠易激综合征大鼠模型。造模成功后，将造模组随机分为模型组和痛泻要方组，每组10只。痛泻要方组大鼠灌胃痛泻要方4.92 g/ml，正常组、模型组灌胃等体积生理盐水，按2 ml/100 g大鼠体重灌胃，1次/d，连续灌胃14 d。采用电子恒压器测定结直肠扩张刺激下腹外斜肌肌电反应，评价内脏敏感性，采用HE染色观察大鼠结肠组织形态，ELISA法检测大鼠结肠中5-羟色胺(5-hydroxytryptamine, 5-HT)水平，qPCR法检测结肠组织中5-羟色胺转运体(serotonin transporter, SERT) mRNA水平，Western blot检测大鼠结肠和下丘脑SERT蛋白表达。结果 与模型组比较，痛泻要方组在球囊扩张压力为60、80 mmHg时，肌电图积分变化率[(179.51±18.26)%比(226.42±25.78)%；(242.13±15.42)%比(306.02±51.51)%]降低(P＜0.05或P＜0.01)，结肠5-HT[(8.85±0.53)ng/mg比(12.25±1.95)ng/mg]水平降低(P＜0.01)，结肠SERT mRNA[(0.85±0.12)比(0.38±0.02)]、蛋白[(0.53±0.11)比(0.36±0.17)]表达升高(P＜0.05或P＜0.01)，下丘脑SERT蛋白[(0.88±0.12)比(0.36±0.13)]表达升高(P＜0.05)。结论 痛泻要方可调节腹泻型肠易激综合征模型大鼠5-HT及SERT表达，从而降低内脏高敏感。
- Abstract:
- Objective To explore the effect of Tongxie-Yaofang on visceral sensitivity in IBS-D and the possible mechanism. Methods Divided 30 male SD rats (one-day old) into normal group (10 rats) and IBS-D model group (20 rats) randomly. IBS-D was induced by the method of neonatal maternal separation and restraint stress. After successful modeling, the IBS-D model group was randomly divided into model group and Tongxie-Yaofang group, with 10 rats in each group. Tongxie-Yaofang group was given Tongxie-Yaofang formula, 4.92 g/ml by gavage, while the normal and model groups were given the same amount of normal saline, rats were gavaged with 2 ml/100 g body weight once a day for 14 days. The electromyography of the exorectus muscle was used to meature colorectal distension and by using electronic constant pressure apparatus to evaluate visceral sensitivity. The morphology of colon by HE staining and Enzyme-linked immunosorbent assay (ELISA) were used to determine the level of colonic 5-hydroxytryptamine (5-HT), qPCR was used to detect the colonic mRNA expression of serotonin transporter (SERT) and Western blot was used to detect SERT expression in colon and hypothalamus. Results Compared with the model group, at the expansion pressure of 60 mmHg and 80 mmHg, the electromyographic response [(179.51 ± 18.26)% vs. (226.42 ± 25.78)%; (242.13 ± 15.42)% vs. (306.02 ± 51.51)%] in Tongxie-Yaofang group was significantly decreased ( P<0.05 or P<0.01). The colonic content of 5-HT was significantly lower than that in the model group [(8.85 ± 0.53) ng/mg vs.(12.25 ± 1.95) ng/mg] (P<0.01), the expression of SERT mRNA (0.85 ± 0.12 vs. 0.38 ± 0.02) and SERT protein (0.53 ± 0.11 vs. 0.36 ± 0.17) in the colon was significantly increased ( P<0.05 or P<0.01), the expression of SERT protein (0.88 ± 0.12 vs. 0.36 ± 0.13) in the hypothalamus was significantly increased ( P<0.05). Conclusion Tongxie-Yaofang could relieve the visceral hypersensitivity, which may be achieved by regulating the 5-HT and SERT expression.
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备注/Memo
（收稿日期：2021-01-22）
更新日期/Last Update:
2021-12-16