Lin Li,Zhou Yudan,Xu Jiaqi,et al.Effect of carpesium abrotanoides petroleum ether fraction on phagocytic function of macrophages and antibody forming cell in mice[J].,2019,41(5):487-490.[doi:10.3760/cma.j.issn.1673-4246.2019.05.012]
天名精石油醚部位对小鼠巨噬细胞吞噬功能和抗体形成细胞的影响
- Title:
- Effect of carpesium abrotanoides petroleum ether fraction on phagocytic function of macrophages and antibody forming cell in mice
- Keywords:
- Carpesium abrotanoides; Macrophages; Immune function; Mice
- 摘要:
- 目的 观察天名精石油醚部位对小鼠巨噬细胞吞噬功能和抗体形成细胞的影响。方法 提取天名精石油醚部位，采用随机数字表法将45只小鼠分为对照组及天名精石油醚提取物低、高剂量组，每组15只。天名精石油醚提取物低、高剂量组分别灌胃天名精石油醚提取物50、100 mg/kg，对照组灌胃等体积生理盐水，连续灌胃10 d。检测小鼠脾脏重量和生长指数，采用巨噬细胞吞噬鸡红细胞实验与溶血空斑实验检测小鼠腹腔巨噬细胞的吞噬功能与抗体形成细胞的数量。结果 与对照组比较，天名精石油醚提取物低、高剂量组小鼠脾脏重量[(192.4±11.49)mg、(204.6±10.59)mg比(117.6±10.89)mg]、生长指数[(6.04±0.54)、(6.06±0.40)比(3.89±0.14)]增加(P＜0.05)，抗体形成细胞数[(1 216.4±94.1)、(1 548.8±86.4)比(361.0±11.7)]、巨噬细胞吞噬百分率[(58.60±2.60)%、(72.0±3.08)%比(35.49±1.64)%]、吞噬指数[(2.01±0.10)、(2.69±0.15)比(0.37±0.06)]增加(P＜0.05)。结论 低、高剂量的天名精石油醚部位可增强小鼠特异性和非特异性免疫功能。
- Abstract:
- Objective To study the effects of carpesium abrotanoides petroleum ether fraction on phagocytic function of macrophages and antibody forming cell in mice. Methods The extract carpesium abrotanoides petroleum ether fraction, forty five mice were divided into the control group and low and high dose groups of carpesium abrotanoides petroleum ether fraction by random number table method, 15 mices in each group. The low-dose and high-dose groups of carpesium abrotanoides petroleum ether fraction were given 50 mg/kg and 100 mg/kg respectively by gavage for 10 days. The spleen weight, growth index, the phagocytosis of macrophages and the number of antibody-forming cells in peritoneal macrophages of mice were detected by chicken erythrocyte phagocytosis test and hemolytic plaque test. Results Compared with the control group, the spleen weight (192.4 ± 11.49 mg, 204.6 ± 10.59 mg vs. 117.6 ± 10.89 mg), the growth index (6.04 ± 0.54, 6.06 ± 0.40 vs. 3.89 ± 0.14), antibody forming (1 216.4 ± 94.1, 1 548.8 ± 86.4 vs.361.0 ± 11.7), phagocytosis percentage of macrophages (58.60% ± 2.60%, 72.0% ± 3.08% vs. 35.49% ± 1.64%), and Phagocytosis index (2.01 ± 0.10, 2.69 ± 0.15 vs. 0.37 ± 0.06) of the groups of low doses and high doses of Carpesium abrotanoides Petroleum ether fraction significantly increased (P<0.05). Conclusions The low doses and high doses of Carpesium abrotanoides Petroleum ether fraction can enhance the mice’s specific and nonspecific immune function,and protect the immune system of mice.
参考文献/References:
［1］ 蒋洪,宋纬文. 中草药实用图典[M]. 福州:福建科学技术出版社,2017:520. ［2］ 湖南省食品药品监督管理局. 湖南省中药材标准(2009年版)[M]. 长沙:湖南科学技术出版社,2010:276. ［3］ 刘翠周,许婧,桂丽萍,等. 北鹤虱的化学成分研究[J]. 药物评价研究,2010,33(3):220-221. ［4］ 朴英花,朴惠顺. 倍半萜类化合物生物活性研究进展[J]. 职业与健康,2012,28(18):2291-2293. ［5］ 乔巍,汪小莞,李晓春,等. 四种倍半萜类化合物广谱抗肿瘤效应的研究进展[J]. 中国生化药物杂志,2016,36(3):5-8. DOI:10.3969/j.issn.1005-1678.2016.03.02. ［6］ Lee SB,Kang K,Lee HJ,et al. The chemopreventive effects of Carpesium abrotanoides are mediated by induction of phase II detoxification enzymes and apoptosis in human colorectal cancer cells[J]. J Med Food,2010,13(1):39-46. DOI:10.1089/jmf.2009. 1157. ［7］ Shi YP,Guo W,Jia ZJ. Germacranolides from Carpesium lipskyi[J]. Planta Med,1999,65(1):94-96. DOI:10.1055/s-2006- 960452. ［8］ 刘富岗,冯素香,杨云,等. 首乌藤低聚糖的分离纯化及其对免疫抑制小鼠免疫功能的影响[J]. 中成药,2017,39(2):390-392. DOI:10.3969/j.issn.1001-1528.2017.02.034. ［9］ 熊平源,王强. 医学免疫学实验教程[M]. 武汉:武汉大学出版社,2011:8-9. ［10］ Wang F,Yang K,Ren FC,et al. Sesquiterpene lactones from Carpesium abrotanoides[J]. Fitoterapia,2009,80(1):21-24. DOI:10.1016/j.fitote.2008.09.009. ［11］ 俞小瑞. 基础医学导论[M]. 北京:人民卫生出版社,2015:220. ［12］ 徐纪茹,吕昌龙. 病原宿主与防御系统[M]. 北京:人民卫生出版社,2016:184.
相似文献/References:
[1]徐明 帅朝霞 陈国千.血必净注射液对脂多糖诱导HMGB1 胞外释放的影响[J].国际中医中药杂志,2009,31(05):393.[doi:10.3760/cma.j.issn.1673-4246.2009.05.003]
XU Ming,SHUAI Zhao-xia,CHEN Guo-qian*.Effect of Xuebijing Injection on Extracellular Release of HMGB1 Induced by Endotoxin[J].,2009,31(5):393.[doi:10.3760/cma.j.issn.1673-4246.2009.05.003]
[2]李森 于新辉.槲皮素对脂多糖刺激巨噬细胞炎症因子释放的影响[J].国际中医中药杂志,2012,34(09):797.[doi:10.3760/cma.j.issn.1673-4246.2012.09.010]
LI Sen,YU Xin-hui.Effect of quercetin on LPS-stimulated macrophage inflammatory cytokine release[J].,2012,34(5):797.[doi:10.3760/cma.j.issn.1673-4246.2012.09.010]
[3]陈国千 王婷婷 胡志刚 王春新 徐明.小檗碱抑制巨噬细胞HMGB1释放及机理研究[J].国际中医中药杂志,2009,31(02):103.[doi:10.3760/cma.j.issn.1673-4246.2009.02.002]
CHEN Guo-qian*,WANG Ting-ting,HU Zhi-gang,et al.Effect of Berberine on HMGB1 Release from Macrophages and its Mechanism[J].,2009,31(5):103.[doi:10.3760/cma.j.issn.1673-4246.2009.02.002]
[4]续畅 赵庆国 肖小河 赵奎君 王伽伯 马致洁.不同产地昆明山海棠对巨噬细胞炎性因子的影响[J].国际中医中药杂志,2015,37(11):1005.[doi:10.3760/cma.j.issn.1673-4246.2015.11.012]
Xu Chang*,Zhao Qingguo,Xiao Xiaohe,et al.The effect of different regions of Tripterygium Hypoglaucum (Lévl.) Hutch on macrophage inflammatory factor[J].,2015,37(5):1005.[doi:10.3760/cma.j.issn.1673-4246.2015.11.012]
[5]续畅 赵庆国 肖小河 赵奎君 王伽伯 马致洁.雷公藤及昆明山海棠对巨噬细胞炎性因子的影响 [J].国际中医中药杂志,2016,38(09):821.[doi:10.3760/cma.j.issn.1673-4246.2016.09.016]
Xu Chang*,Zhao Qingguo,Xiao Xiaohe,et al.The effect of Tripterygium Wilfordii Hook. F. and Tripterygium Hypoglaucum ( Lévl. ) Hutch on macrophage inflammatory factor [J].,2016,38(5):821.[doi:10.3760/cma.j.issn.1673-4246.2016.09.016]
[6]杨椹 冯俊霞 段燕灵 张云芳 李红艳.黄芪甲苷对小鼠RAW264.7巨噬细胞缺氧复氧损伤的影响[J].国际中医中药杂志,2017,39(08):714.[doi:10.3760/cma.j.issn.1673-4246.2017.08.011]
Yang Shen,Feng Junxia,Duan Yanling,et al.Protective effect of Astragaloside Ⅳ on the oxygenation induced by RAW264.7 murine macrophage [J].,2017,39(5):714.[doi:10.3760/cma.j.issn.1673-4246.2017.08.011]
[7]张思望 葛晓静 胡立宏 韩婧.蒙古苍耳草提取物抗屋尘螨致小鼠过敏性哮喘作用研究[J].国际中医中药杂志,2021,43(01):37.[doi:10.3760/cma.j.cn115398-20200615-00157]
Zhang Siwang,Ge Xiaojing,Hu Lihong,et al.Study on the effect of Xanthium mongolicum Kitag. extract on allergic asthma induced by house dust mite in mice[J].,2021,43(5):37.[doi:10.3760/cma.j.cn115398-20200615-00157]
备注/Memo
通信作者：王强，Email: 251204399@qq.com，电话：15971509779 基金项目：湖北省科技厅自然科学基金(2017CFB517)；武汉科技大学大学生科技创新基金研究项目(17ZRC79)
更新日期/Last Update:
2019-05-08