LIU Yuan-xin*,LIU Ting,YANG Xiao-yan,et al.Effects of Angong-niuhuang pill on expression of eNOSmRNA in spontaneously hypertensive rats after intracerebral hemorrhage[J].,2011,33(02):107-110.[doi:10.3760/cma.j.issn.1673-4246.2011.02.004]
安宫牛黄丸对自发性高血压大鼠脑出血后内皮型一氧化氮合酶信使核糖核酸表达的影响
- Title:
- Effects of Angong-niuhuang pill on expression of eNOSmRNA in spontaneously hypertensive rats after intracerebral hemorrhage
- Keywords:
- Angong-niuhuang Pills; Intracerebral hemorrhage; eNOSmRNA
- 文献标志码:
- A
- 摘要:
- 目的 观察安宫牛黄丸对自发性高血压大鼠脑出血后内皮型一氧化氮合酶信使核糖核酸（eNOSm RNA）表达的影响，探讨其保护作用机制。方法 采用自体血制作大鼠脑出血模型，将120只自发性高血压大鼠按随机数字表法分为模型组、西药治疗组、安宫牛黄丸组、中西药结合组。应用RT-PCR法测定用药后eNOSmRNA表达的变化。结果 中西结合组eNOSmRNA表达明显增高（P＜0.01），安宫牛黄组、西药治疗组eNOSmRNA表达明显高于模型组（P＜0.01），安宫牛黄丸组与西药治疗组间相比差异无统计学意义（P＞0.05）。结论 安宫牛黄丸对自发性高血压大鼠脑出血后的治疗作用可能与eNOS的表达有关。
- Abstract:
- Objective To study the effect of Angong-niuhuang pill on expression of eNOSmRNA in spontaneously hypertensive rats after intracerebral hemorrhage and to explore it’s protective mechanism. Methods 120 spontaneously hypertensive rats were randomly divided into four groups: model group, western medicine treated group. Angong-niuhuang pill treated group, combining traditional Chinese medicine western medicine group, expression of eNOSmRNA in rat brain tissue was detected by RT-PCR. Results Expression of BCL-2mRNA in combining traditional Chinese medicine western medicine group was significantly higer than other goups(P＜0.01), Compared with model group, the expression of BCL-2mRNA in western medicine treated group and Angong-niuhuang pill treated group was markedly increased (P＜0.01), the differences between Angong-niuhuang pill treated group and Western medicine treated group were not obvious (P＞0.05). Conclusion Protective effect of Angong-niuhuang pill on spontaneously hypertensive rats after intracerebral hemorrhage is related to the the inducement of eNOS expression.
参考文献/References:
［1］ Qureshi AI，Tuhrim S，Broderick JP，et al. Spontaneous intracerebral Hemorrhage. N Engl J Med，2001，344（19）：1450-1460.
［2］ Flaherty ML，Haverbusch M，Sekar P，et al. Long-term mortality after intracerebral hemorrhage. Neurology，2006，66（8）：1182-1186.
［3］ Samanta J， Alden T， Gobeske K，et al. Noggin protects against ischemic brain injury in rodents. Stroke，2010，41（2）：357-362.
［4］ Qureshi AI，Mendelow AD，Hanley DF. Intracerebral haemorrhage. Lancet，2009，373（9675）：1632-1644.
［5］ Rosenberg GA，Mun-Bryce S，Wesley M，et al. Collagenase-induced intracerebral hemorrhage in rats. Stroke，1990，21（5）：801-807.
［6］ 包新民，舒斯云. 大鼠脑立体定位图谱. 北京：人民卫生出版社，1991：35.
［7］ Longa EZ，Weinstein PR，Carlson S，et al. Reversible middle cerebral artery occlusion without cranectomy in rat. Stroke，1989，20（10）：84-91.
［8］ Ratliff B，Rodebaugh J，Sekulic M，et al. Nitric oxide synthase and renin-angiotensin gene expression and NOS function in the postnatal renal resistance vasculature. PediatrNephrol，2009，24（2）：355-365.
［9］ Shah AM，MacCarthy PA. Paracrine and autocrine effects of nitric oxide on myocardial function. Pharmacol Ther，2000，86（1）：49-86.
［10］ DING Wen-gang，ZHOU Hua-cheng，CUI Xiao-guang，et al. Anti-apoptotic effect of Morphine-induced delayed preconditioning on pulmonary artery endothelial cells with anoxia/reoxygenation lnjury. Chinese Medical Journal，2008，121（14）：1313-1318.
［11］ Ma SY，Xiang JZ，WU JL，et al. Endogenous nitric oxide mediates lipoteichoic acid induced preconditioning on reoxygenation injury of cultured human coronary artery endothelial cells. Acta Pharmaceutica Sinaca（Chin），2005，40（4）：316-321.
［12］ Kaminski A，Kasch C，Zhang L，et al. Endothelial nitric oxide synthase mediates protective effects of hypoxic preconditioning in lungs. Respir Physiol Neurobiol，2007，155（3）：280-285.
［13］ Koti RS，Tsui J，Lobos E，et al. Nitric oxide synthase distribution and expression with ischemic preconditioning of the rat liver. FASEB J，2005，19（9）：1155-1157.
相似文献/References:
[1]高丽丽 吴成翰.脑出血后胰岛素样生长因子-1表达与中风闭、脱证关系的研究[J].国际中医中药杂志,2012,34(03):207.[doi:10.3760/cma.j.issn.1673-4246.2012.03.005]
GAO Li-li,WU Cheng-han.Study on relationship among expression of IGF-1 in peri-hematomal brain region, excessive and depletion syndrome of stroke after cerebral hemorrhage[J].,2012,34(02):207.[doi:10.3760/cma.j.issn.1673-4246.2012.03.005]
[2]韩培军 张瑞花 辛振学.熄风化痰祛瘀法辅助手术治疗脑出血41例的疗效观察[J].国际中医中药杂志,2009,31(05):423.[doi:10.3760/cma.j.issn.1673-4246.2009.05.022]
HAN Pei-jun,ZHANG Rui-hua,XIN Zhen-xue.Therapeutic Effects Observation on Treatment of 41 Cases of Cerebral Hemorrhage with Operation and Chinese Medicines of Stopping Endogenous Wind, Eliminating Phlegm and Removing Blood Stasis[J].,2009,31(02):423.[doi:10.3760/cma.j.issn.1673-4246.2009.05.022]
[3]李娜 甄文剑 文博.穴位电刺激治疗脑出血术后尿失禁临床研究[J].国际中医中药杂志,2017,39(09):781.[doi:10.3760/cma.j.issn.1673-4246.2017.09.004]
Li Na*,Zhen Wenjian,Wen Bo..Observation of curative effect of point electric stimulation for urinary incontinence after postoperative cerebral hemorrhage[J].,2017,39(02):781.[doi:10.3760/cma.j.issn.1673-4246.2017.09.004]
[4]王东雁 刘杰 陈敬德 王明 王勇 邵文泽 王蒙 李辉. 电针与穴位注射联合康复技术对脑出血后肩手综合征患者上肢功能障碍及日常生活能力的影响[J].国际中医中药杂志,2018,40(7):600.[doi:10.3760/cma.j.issn.1673-4246.2018.07.006]
Wang Dongyan*,Liu Jie,Chen Jingde,et al.Therapeutic effect of acupoint injection and rehabilitation training program on upper limb function and daily life activities of patients with shoulder-hand syndrome after cerebral hemorrhage [J].,2018,40(02):600.[doi:10.3760/cma.j.issn.1673-4246.2018.07.006]
[5]周之煜 王冠 王建业 董琨.针刺辅助抢救超早期脑疝危象患者2例[J].国际中医中药杂志,2020,42(03):284.[doi:10.3760/cma.j.cn115398-20190102-00005]
[J].,2020,42(02):284.[doi:10.3760/cma.j.cn115398-20190102-00005]
备注/Memo
基金项目：国家自然科学基金项目（项目编号：30760315）
更新日期/Last Update: